Characterization of the interaction of cytochrome c and mitochondrial ubiquinol-cytochrome c reductase.
نویسندگان
چکیده
منابع مشابه
The interaction of arylazido ubiquinone derivative with mitochondrial ubiquinol-cytochrome c reductase.
An arylazido ubiquinone derivative, 2,tdimethoxy5-methyl-6{10-[4-(azid0-2-nitroanilinopropionoxy)]decyl} -1,4-benzoquinone, restores about 60% of the electron transfer activity to ubiquinoneand phospholipid-depleted succinateor ubiquinol-cytochrome c reductases, compared to that restored by 2,3-dimethoxy5-methyl-6-(1O-bromodecyl)-1,4-benzoquinone. The restored activity is fully sensitive to ill...
متن کاملStructure and function of the mitochondrial ubiquinol: cytochrome c reductase and NADH: ubiquinone reductase.
Structurc of' uhiquinol : cytochrome c reductase Ubiquinol : cytochrome c reductase (cytochrome reductase) from Neurospora crassa mitochondria is a dimer and the monomeric unit consists of nine different subunits. The subunits I and I I are known as core-proteins, I I I , IV and V are the cytochromes h, c , and the iron-sulphur protein, and VI to IX are small proteins without prosthetic groups ...
متن کاملQuantitative resolution of succinate-cytochrome c reductase into succinate-ubiquinone and ubiquinol-cytochrome c reductases.
A purified, active succinate-ubiquinone reductase was prepared from succinate-cytochrome c reductase without damage to ubiquinol-cytochrome c reductase by 1.1% Triton X-100 solubilization at pH 8.0, and calcium phosphate column chromatography in 50 mM Tris-succinate buffer, pH 8.0, containing 30 mM potassium phosphate. Succinate-ubiquinone reductase thus obtained contains ubiquinone and catalyz...
متن کاملCytochrome c-mediated electron transfer between ubiquinol-cytochrome c reductase and cytochrome c oxidase. Kinetic evidence for a mobile cytochrome c pool.
Ubiquinol oxidase has been reconstituted from ubiquinol-cytochrome c reductase (Complex III), cytochrome c and cytochrome c oxidase (Complex IV). The steady-state level of reduction of cytochrome c by ubiquinol-2 varies with the molar ratios of the complexes and with the presence of antimycin in a way that can be quantitatively accounted for by a model in which cytochrome c acts as a freely dif...
متن کاملInteraction and identification of ubiquinone-binding proteins in ubiquinol-cytochrome c reductase by azido-ubiquinone derivatives.
Various azido-ubiquinone derivatives were synthesized and characterized. 3-Azido-2-methyl-5-methoxy-6-(3,7-dimethyloctyl)-1,4-benzoquinone was found to be suitable for the study of specific interaction between ubiquinone (Q) and protein. It was synthesized with high specific radioactivity and used to identify the Q-binding proteins in purified ubiquinol-cytochrome c reductase. This azido-Q deri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43566-6